Tweedy knew they would eventually need to face tough questions from a rogue music journalist.
Appearing on Monday night's episode of The Colbert Report, the father-son duo were not spared the hard-hitting queries.
"Do you think you two could take Billy Ray and Miley Cyrus in a fight?" host Stephen Colbert asked.
Spencer Tweedy wasted no time answering, "Absolutely not."
So it went for a couple minutes before Jeff Tweedy (Wilco's frontman) and Spencer Tweedywere able to kick out the jams and charm the live audience with "Low Key."
Check out the interview here:
Tweedy perfoms "Low Key" in this clip:
The band also did a special Internet-only performance of "World Away":
Tweedy's backstory is more interesting than just "father and son decide to play rock together."
As previously reported, Susan Miller Tweedy, Jeff Tweedy's wife and Spencer Tweedy's mother, was diagnosed with a rare type of non-Hodgkin's lymphoma in April and underwent months of chemotherapy to battle the disease.
But a local school has helped raise thousands of dollars to support Susan Miller Tweedy's "Light the Night" team, which is participating in a fundraising walk next month to raise money for research by the Leukemia and Lymphoma Society.
While Susan Tweedy's prognosis is good as far as the lymphoma goes, she is currently waiting for results from a bone scan to see if the chest cancer she beat 22 years ago has come back.
Sukierae, the upcoming album from Tweedy, was inspired by Jeff Tweedy's attempts to come to grips with his wife's illness in a positive way. When he talked to Rolling Stone earlier this month, he shared that "Sukierae" is Susan Tweedy's nickname.
Spencer Tweedy, who put off going to Wisconsin's Lawrence University for a year in order to be with his family, said, "Mom is kind of the center of everything. I have no problem enshrining my mother in an album. It makes sense. She's No. 1 in the whole operation."